نتایج جستجو برای: ژن مقاومت Pto
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مقاومت گیاهان در برابر دامنه وسیعی از بیماریهای گیاهی بستگی به ژن غیر بیماریزایی درعامل بیماری و ژن مقاومت در میزبان دارد. در این تحقیق ژن غیر بیماریزایی avrptoجدایه pt23 باکتری pseudomonas syringae pv.tomato در پلاسمید نوترکیبpla(avrpto) کلون گردیدوسپس این پلاسمیدازطریق الکتروپوریشن به داخل سلولهای جدایه های وحشی p.s. pv tomato. ، p.s.pv. phaseolicolaو p.s.pv. syringae منتقل شد. رقم حساس کانری...
وراثت مقاومت سفیدبالک گلخانه، Trialeurodes vaporariorum Westwood (Hemiptera: Aleyrodidae)، به دو ترکیب ایمیداکلوپرید و کلرپایریفوس مورد مطالعه قرار گرفت. در این پژوهش، از جمعیت فیلستان ورامین (FL) عنوان والد مقاوم فردیس کرج (FR) حساس استفاده شد. مقدار ترتیب حدودا 62/13 91/14 برابر بود. عدم وجود اختلاف معنیدار LC50 روی تلاقیهای F1 (R♂×S♀) Fʹ1 (R♀×S♂) نشان داد که T. نوع اتوزومی (غیرجنسی) است. ه...
Resistance to bacterial speck disease in tomato (Solanum lycopersicum) is activated upon recognition by the host Pto kinase of either one of two sequence-unrelated effector proteins, AvrPto or AvrPtoB, from Pseudomonas syringae pv tomato (Pst). Pto induces Pst immunity by acting in concert with the Prf protein. The recently reported structure of the AvrPto-Pto complex revealed that interaction ...
Pto confers disease resistance to Pseudomonas syringae pv tomato carrying the cognate avrPto gene. Overexpression of Pto under the cauliflower mosaic virus 35S promoter activates spontaneous lesions and confers disease resistance in tomato (Lycopersicon esculentum) plants in the absence of avrPto. Here, we show that these AvrPto-independent defenses require a functional Prf gene. Several Pto-in...
MicroRNAs (miRNAs) regulate gene expression in many biological processes, but the significance of the interaction between a miRNA and its targets in perennial trees remains largely unknown. Here, we employed transcript profiling and association studies in Populus tomentosa (Pto) to decipher the effect of genetic variation and interactions between Pto-miR156c and its potential targets (Pto-SPL15...
The Pto gene was derived originally from the wíld tomato species Lycopersicon pimpinellifolium and confers resistance to Pseudomonas syringae pv tomato strains expressing the avirulence gene avrPto. The Fen gene is also derived from L. pimpinellifolium and confers sensitivity to the insecticide fenthion. We have now isolated and characterized the alleles of Pto and Fen from cultivated tomato, L...
Cyanobacteria are the only model circadian clock system in which a circadian oscillator can be reconstituted in vitro. The underlying circadian mechanism appears to comprise two subcomponents: a post-translational oscillator (PTO) and a transcriptional/translational feedback loop (TTFL). The PTO and TTFL have been hypothesized to operate as dual oscillator systems in cyanobacteria. However, we ...
MicroRNAs (miRNAs) play crucial roles in plant growth and development, but few studies have illuminated the allelic interactions among miRNAs and their targets in perennial plants. Here, we combined analysis of expression patterns and single-nucleotide polymorphism (SNP)-based association studies to explore the interactions between Pto-MIR475b and its four target genes (Pto-PPR1, Pto-PPR2, Pto-...
Tomato (Lycopersicon esculentum) Pto kinase specifically recognizes the Pseudomonas effector proteins AvrPto and AvrPtoB, leading to induction of defense responses and hypersensitive cell death. Structural modeling of Pto combined with site-directed mutagenesis identified a patch of surface-exposed residues required for native regulation of signaling. Mutations in this area resulted in constitu...
Mycophenolic acid (MPA) is the active metabolite of mycophenolate mofetil. This study designed to investigate the mechanism of cytotoxicity of MPA on the genetically engineered PC12 Tet Off (PTO) neuronal cells with p53 gene. Alamar Blue (AB) reduction showed concentration-dependent cytotoxicity of MPA on PTO cells with IC50 value of 32.32 ± 4.61 mM. The reactive oxygen species (ROS) generation...
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